Fig. 5.
Killing of K562 and ML-2 cells by NK lysates, streptolysin O, and complement.
K562 and ML-2 cells were incubated with 100 μg total protein of the NK lysates, 5 U/μL streptolysin O, or 10 U/mL complement for 30 minutes at 37°C. Dead cells were stained with 150 μL of the PI solution and analyzed by flow cytometry.