Requirement for the C/EBP binding site for activation of the G-CSFR promoter by AML1-MTG8.

(A) Schematic representation of the base substitution of the mutants around the C/EBP site in the G-CSFR promoter. GRP16M1 is a mutant of the C/EBP consensus site. GRP16M2 is a mutant of the putative Sp1 site. GRP16M3 is a mutant of the Sp1 consensus site. (B) Effects of AML1-MTG8 on the activity of GRP16 mutants in L-G cells. Cells were cotransfected with each mutant reporter and either LNSX, LNSX-AML1b, or LNSX-AML1-MTG8.