Fig. 6.
Induced expression of G-CSFR and growth stimulation by C/EBPε.
(A) Expression of C/EBPε protein in L-G cells detected by Western blot analyses. Proteins from the L-G infectants with viruses encoding AML1b, AML1-MTG8, or C/EBPε were analyzed for immunodetection using anti-HA antibody. The positions of AML1b, AML1-MTG8, and C/EBPε are indicated by arrows. (B) Total RNAs (5 μg of RNA) were prepared from L-G infectants cultured with IL-3, blotted, and hybridized with32P-labeled mouse G-CSFR cDNA, mouse MPO cDNA, and human G3PDH cDNA. (C) Growth curves of L-G cells infected with LNSX retroviruses carrying human C/EBPε cDNA or AML1-MTG8 cDNA in the presence of G-CSF.
