Overexpression of G-CSFR stimulates cell proliferation in response to G-CSF.

(A) Growth curves of L-G cells infected with LNSX retroviruses carrying mouse G-CSFR cDNA or AML1-MTG8 cDNA in the presence of G-CSF. (B) Correlation between G-CSFR activity and the rate of proliferation in the presence of G-CSF. The 32Dcl3 transfected cells were inoculated into medium supplemented with 10 ng/ml of G-CSF at a cell density of 2 × 10⁴ cells/mL, and cell numbers were counted with a Coulter counter after 5 days of incubation. (C) Cell morphology of G-CSFR transformed GR8 cells when cultured with G-CSF for 10 days. GR8 is a representative clone that expresses G-CSFR at a high level. Cells were stained with May-Gruenwald-Giemsa solution.