HIV-1 infection of homozygous ccr5▵32 PBLs.

We stimulated ccr5Δ32 PBLs with PHA/IL-2 and infected with approximately 1200 TICD₅₀ units of M6-v3 in the presence or absence of the indicated amounts of antibodies or the CXCR4 antagonist (AMD3100). The cells were stained for cell surface STRL33 and intracellular p24 antigen 1 week after infection. The p24-antigen–positive gate was defined as the region giving less than 0.01% of p24-antigen–positive cells when the p24-antigen antibody was used on uninfected donor cells (negative control). The p24-antigen–positive cells are indicated in red. (A) Upper panel: The percent of p24-antigen–positive cells in the presence or absence of the indicated antibodies or AMD3100 are shown in their respective dot-plots. Lower panel: The distribution of p24-antigen–positive cells in the STRL33⁺ or STRL33⁻ gates under the same conditions. (B) Lower panel: Graphic representation of part A. The total number of p24-antigen–positive cells in the presence or absence of AMD3100 or the nonneutralizing anti-STRL33 antibody was normalized to 100%, and the proportion of p24-antigen–positive cells in the STRL33⁺ or STRL33⁻ gates are shown.