Fig. 2.
Northern blot analysis of eIF3-p44 in various human tissues.
Blot filters containing 2 μg poly (A)+RNA were hybridized with a 32P-labeled cDNA fragment (0.9 kb) encoding amino acid residues 54-321 of human eIF3-p44. The same blot was stripped and reprobed with β-actin to quantify RNA loading. eIF3-p44 mRNA is abundantly expressed in human tissues including testis, skeletal muscle, pancreas, and heart.