Fig. 1.
Northern blot analysis of Trf expressed by wild-type and mutant animals.
Total liver RNA from homozygous, heterozygous, and wild-type animals was analyzed by Northern blot with a probe derived from the 5′ end of mouse Trf cDNA. All samples show hybridization to a 2.5-kb message, as expected for the full-length wild-type Trftranscript. The Trfhpx/+ animal shows approximately 50% as much signal as wild-type animals. However, very little Trf transcript was detected inTrfhpx/hpx mice. The lower panel shows the same blot after being stripped and reprobed with radiolabeled β-actin cDNA to demonstrate equal loading of the lanes.
