Fig. 3.
![Fig. 3. Pulse-chase analysis of rVWFR273W secretion from COS-7 cells. / COS-7 cells were transfected with the appropriate plasmid DNA. Forty-eight hours after transfection, the cells were radiolabeled for 20 minutes with [35S]-methionine and [35S]-cysteine, then chased for various times in the presence of nonradioactive methionine and cysteine. Radiolabeled cell lysates and medium samples were immunoprecipitated with a rabbit polyclonal antiserum to VWF. Immunoprecipitates were separated by electrophoresis on 6% reducing SDS-polyacrylamide gels. Upper bar denotes the position of pro-VWF, lower bar denotes the position of mature propeptide cleaved VWF.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/2/10.1182_blood.v96.2.560/5/bloo01401003w.jpeg?Expires=1736046084&Signature=KDQS7zavrZ8JzuIs-nsVK483dX9-CSh3GxiUMLDPJmHH4gsPwCSDr-5xaXjCkR8WsQ1Lltty21BBv8hDoPa~82VQ95TegWKd3qSJO4Dji30KpCsQpY2pEMPWBiUn6IsxQ2g3XOtqcsqNaJWEB--1~Kr4AmRvINjUOnBg3w8OpUIVwKkHiGzjRopcUULLhW0Hez7qpzDqHRu9ZuafzoRF8yblmX-08DRZedJL70br4wNy97jVssvauYONCcl4v8CtYiqnpNPPcxEzMfuyRQWZTkPHth7GVqJbJgtggTuzJVzkGgGH3VHk~YfnRC176KB~txTixa~oZhF87d5x0Ji6rA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of rVWFR273W secretion from COS-7 cells.
COS-7 cells were transfected with the appropriate plasmid DNA. Forty-eight hours after transfection, the cells were radiolabeled for 20 minutes with [35S]-methionine and [35S]-cysteine, then chased for various times in the presence of nonradioactive methionine and cysteine. Radiolabeled cell lysates and medium samples were immunoprecipitated with a rabbit polyclonal antiserum to VWF. Immunoprecipitates were separated by electrophoresis on 6% reducing SDS-polyacrylamide gels. Upper bar denotes the position of pro-VWF, lower bar denotes the position of mature propeptide cleaved VWF.
