Restriction maps of the GRAP gene, theGRH gene, and related constructs.

Genes and constructs are described in “Materials and methods,” and the direction of transcription in all maps is from left to right. (A) GRAP gene-coding region is shown in solid shading. GAD extends from residues 768 to 881. Both pGAD-GRAP and pEGFP-GRAP are fused to GRAP at codon 126. (B) GRH gene-coding region is shown in the shaded striped bar. URA3 gene is shown in the striped bar.