Gene delivery in HC monocytes–macrophages bySalmonella carrier.

In vitro differentiated macrophages from patients with HC were infected with Salmonella harboring the pCMV-βgal plasmid. (A) βgal expression in peripheral blood mononuclear cells was restricted to macrophages (large cells) and spared lymphocytes (small cells). (B) A duplicate cell preparation of A after complement-lysis of lymphocytes showing high βgal expression in most cells.