Semiquantitative Cx mRNA expression in stromal cell lines.

The experiment shows the expression of Cx30.3 (182 bp, A), Cx31 (182 bp, B), Cx31.1 (357 bp, C), Cx43 (294 bp, D), and Cx45 (444 bp, E) in 8 different stromal cell lines. Rabbit α-globin was used as an internal standard (257 bp). HPRT was used as a housekeeping control gene (179 bp, F). Analyses were performed at least twice. Lane 1, FLB6-1; lane 2, FLB6-2; lane 3, FBMD-1; lane 4, NBM11F4G; lane 5, FL1E2C; lane 6, FL7H7B; lane 7, NBM11F10H; and lane 8, NBM6D3D.