CX43 expression in FL stromal cells.

(A) Cx43 expression in the cell lines Cx43⁺ (FLB6-3 and FLB6-4, from Cx43-KO mice in which the Cx43 gene has been reintroduced). Note that cells show a great variance in their protein expression. Note that the fluorescence (similarly as in Cx43-wt cells, see Figure 2) is distributed along the membrane and the perinuclear area. (B) Western blot analysis of FL stromal cells. FLB6-1 (Cx43-wt) was chosen as Cx43-highly expressing cell line for comparison with Cx43⁻ cells (FLB6-2) showing some background and Cx43⁺ cell lines (FLB6-3 and FLB6-4, representing 23.7% and 9.1% of the band density of FLB6-1, after background subtraction—FLB6-2).