Frequency of various CAFC-week types after coculture with different stromal cell lines.

FLB6-2 (Cx43⁻) is represented by circles; fetal liver-derived FLB6-3 (Cx43⁺), by squares and FLB6-4 (Cx43⁺), by triangles. (A) When normal mouse BM cells were used for the coculture, the mean CAFC frequencies (per 10⁵ cells) for weeks 1 to 4 were 36.06, 17.10, 2.10, and 1.05 (with the cell line FLB6-2); 40.0, 13.8, 1.7, and 0.8 (with the cell line FLB6-4); 61.7, 17.5, 2.8, and 1.1 (with the cell line FLB6-3). (B) When 5-FU–treated mouse BM cells were cocultured, the mean CAFC frequencies (per 10⁵ cells) were 29.3, 28.7, 3.1, and 0.5 (with the cell line FLB6-2); 32.7, 30.6, 9.8, and 1.3 (with the cell line FLB6-4); and 54.9, 17.1, 8.0, and 3.1 (with the cell line FLB6-3). Four experiments run in parallel were carried out for the different stromal cell lines except for the analysis of CAFC frequency of normal BM on the cell line FLB6-4 (n = 2). *P < .05 between CAFC support by FLB6-2 and FLB6-3, #P < <.05 between CAFC support by FLB6-2 and FLB6-4, ^P < .05 between CAFC support by FLB3 and FLB6-4.