Isolation of E11-5 by cDNA RDA.

(A) The right lane contains EML C1 cDNA representation. The left lane contains the φχ174/HaeIII markers. (B) The result of EML C1-MPRO cDNA RDA was resolved on 2% agarose and stained with ethidium bromide. Some bands contained more than one DNA species. (C) Northern blot analysis of the expression of E11-5 in cell lines representing different hematopoietic lineages. Each lane contains 5 μg total RNA from NIH3T3 (fibroblast), MPRO (neutrophil), J774 (macrophage), EML C1 (stem cell-like), MC/9 (mast cell), FLDS-19 (erythroblast), BaF3 (B-cell), and EL-4 (T-cell). The blot was hybridized with a radiolabeled, nick-translated E11-5 (mJagged2) probe.