Inhibition of colony formation by transient overexpression of WT or DM BAD.

(A) Parental 32D cells were cocultured with LXSP-, WT HA-BAD/LXSP–, or DM HA-BAD/LXSP–transfected BOSC 23 cells. After 48 hours, infected cells were plated in methylcellulose in the presence of IL-3 and puromycin (2.5 μg/mL). Colonies were counted 10 days later. The effect of BAD on the clonogenic capacity of infected cells is expressed as percentage of inhibition of the clonogenic ability of vector-infected cells. (B) BCR/ABL-expressing cells were cocultured with LXSP-, WTHA-BAD/LXSP–, or DMHA-BAD/LXSP–transfected BOSC 23 cells and plated in methylcellulose in the presence of puromycin (2.5 μg/mL). The percentage of inhibition of clonogenic activity (mean + SD) in the presence (□) or absence (▪) of IL-3 is representative of 3 independent experiments.