American Society of Hematology

Fig. 3.

$Fig. 3. Western blots. / (A) Expression of ectopic HA-BAD in selected clones of parental and BCR/ABL-expressing cells. The Western blot shows expression of WT and DM BAD proteins in representative clones. Levels of HSP90 were detected as control for protein loading. (B) WT and DM BAD expression levels in subcellular fractions of infected parental (32D WT BAD) and BCR/ABL-expressing (BCR/ABL WT and DM BAD) cells. Western blots show HA-BAD levels in fractions enriched for mitochondria (HM), and cytoplasm (C) from infected parental and BCR/ABL-expressing cells. Levels of the mitochondrial marker subunit IV of cytochrome oxidase (COX) and cytoplasmic marker HSP90 were measured as control of equal loading. Results are representative of 3 independent experiments.$

Western blots.

(A) Expression of ectopic HA-BAD in selected clones of parental and BCR/ABL-expressing cells. The Western blot shows expression of WT and DM BAD proteins in representative clones. Levels of HSP90 were detected as control for protein loading. (B) WT and DM BAD expression levels in subcellular fractions of infected parental (32D WT BAD) and BCR/ABL-expressing (BCR/ABL WT and DM BAD) cells. Western blots show HA-BAD levels in fractions enriched for mitochondria (HM), and cytoplasm (C) from infected parental and BCR/ABL-expressing cells. Levels of the mitochondrial marker subunit IV of cytochrome oxidase (COX) and cytoplasmic marker HSP90 were measured as control of equal loading. Results are representative of 3 independent experiments.

This Feature Is Available To Subscribers Only