Expression levels of proteins and mRNA.

(A) Expression levels of BCL-2 and BCL-X_Lproteins in total extracts from clones of WT BAD- and DM BAD-infected BCR/ABL-expressing cells. Cell extracts were blotted with anti-BCL-2, anti-BCL-X, and anti-HA antibodies. Levels of HSP90 were analyzed as control of equal loading. Lane C indicates extracts from vector-infected BCR/ABL-expressing cells. (B) Analysis of bcl-2and bcl-x mRNA levels in clones of WT BAD- and DM BAD-infected BCR/ABL-expressing cells. Northern blots were hybridized with probes for bcl-2 and bcl-x. GAPDH mRNA levels (bottom panel) were also measured as control of loading.