In vitro DNase I footprinting of the human ankyrin-1 promoter.

In vitro DNAse I footprinting of the human ankyrin-1 gene promoter was performed using erythroid (K562) extracts. Lane 1 reaction mixture does not contain nuclear extracts. Lanes 2, 3, and 4 contain nuclear extracts and increasing amounts of DNaseI. (See text for details.) A protected site, GCCGATAAG, corresponding to a GATA-1 consensus binding site was identified. In additional experiments, a second protected site, GCCACCCCTCCGCCC, corresponding to binding motifs for Sp1 and CACCC-related proteins was identified in the upstream sequence (not shown).