Unresponsiveness to the recipient mHAg B6^dom1 is not reversed by post-transplant tumor immunization of recipients.

One month after BMT, allogeneic BMT recipients or control C3H.SW donors were immunized twice with irradiated 205IL-2/TK cells at a 1-week interval. Ten days after the second vaccine, their splenocytes were stimulated for 5 days in vitro with B6^dom1-loaded C3H.SW spleen cells. A ⁵¹Cr-release assay was then performed using C3H.SW ConA lymphoblast targets that were either loaded with B6^dom1 peptide (B6^dom1/SW CAB) or not loaded with peptide (SW CAB). Data shown are at an E:T ratio of 50:1, and each effector:target condition was performed in triplicate using splenocytes pooled from 2 or 3 mice.