Analysis of the morphologic characteristics of M1 and M1E2F-1 cells before and after treatment with IL-6.
(A) Representative photomicrographs of May-Grünwald-Giemsa–stained cytospin smears of cells are shown (magnification, ×400). Cells were seeded at 0.1 × 106/mL and treated with IL-6 (50 ng/mL) for 3 days before they were collected for analysis. (B) Cell-type distribution of M1 and M1E2F-1 cells at 3 days after IL-6 stimulation. Morphologic differentiation was determined by counting at least 300 cells on stained cytospin smears, and scoring the proportion of immature blast cells, cells at intermediate stages of differentiation, and mature macrophages. For each cell type, analysis was performed several times with similar results. The data represent a mean value obtained from at least 3 independent determinations and have a standard deviation of ± 15%. Furthermore, there was no difference in the response of the parental M1 cells and the control M1-puromycin cells transfected with the empty puromycin vector.
