Comparison of LEC and RANTES.

(A) Comparison of LEC and RANTES-induced CCR1/HEK-293 adhesion. (B) Comparison of LEC and I309-induced CCR8/HEK-293 adhesion. (C) Comparison of I309 and RANTES-induced THP-1 adhesion. (D) Demonstration of LEC-induced THP-1 adhesion and inhibition by anti-LEC. Receptor transfected cells were incubated for 30 minutes with varying concentrations of chemokines and antibodies. LEC-induced response is indicated with solid black bars, RANTES or I309-induced response is indicated with right-hand hatched bars. Treatment with LEC (1000 ng/mL) and 20 μg/mL anti-LEC is indicated by solid gray bars, treatment with RANTES (100 ng/mL) and 20 μg/mL anti-LEC is indicated by solid white bars. Preimmune serum treatment is indicated by a horizontal striped bar. Representative of 3 experiments.