Inhibition of LPS-induced up-regulation of CD80, CD83, and CD86.

Bar charts showing the expression of various cell surface markers on MoDCs. Immature MoDCs were split on day 7 of culture and exposed to either diluent control, LPS (100 ng/mL), SB203580 (40 μmol/L), or SB203580 together with LPS for 48 hours, before incubation with fluorochrome-conjugated antibodies directed against cell surface antigens and analysis using a flow cytometer. The data shown are the product of the percentage of cells expressing various cell surface antigens and the mean cell fluorescence of the whole population of cells under scrutiny. The value obtained for cells exposed to LPS alone has been normalized to 1. Error bars indicate the SEM. (A) The LPS-induced up-regulation of CD80, CD83, and CD86 is inhibited by the p38SAPK inhibitor SB203580. Representative single-parameter histograms show the expression of CD80, CD83, and CD86 by day-7 MoDCs 48 hours after the addition of LPS (shaded gray), SB203580 plus LPS (shaded black), or nothing (unshaded). (B) Inhibiting p38SAPK has little or no effect on the LPS-induced changes with regard to CD1a, CD40, HLA-DR, and macropinocytosis.