The role of kininogen on integrin-mediated endothelial cell adhesion.

(A) The adhesion of BRECs to VN-, fibronectin (FN)- and FBG-coated wells was analyzed in the absence (−) or in the presence of the competitors HK, HKa, or cRGDfV (10 μg/mL each), 100 nmol/L PAI-1, or 10 mmol/L ethylenediaminetetraacetic acid as indicated. The extent of cell adhesion is presented as absorbance at 590 nm. Data are mean ± SEM (n = 3) of a typical experiment, and similar results were obtained in at least 3 separate experiments. (B) The effect of HK, HKa, cRGDfV (10 μg/mL each), or 20 μg/mL anti-VN mAb 13H1 on the adhesion of BREC to VN was studied according to 2 different protocols: (a) Cells and competitors were added simultaneously to the VN-coated wells (filled bars), and (b) VN-coated wells were blocked and then incubated for 1 hour with the different competitors followed by extensive washing and addition of cells (hatched bars). The extent of cell adhesion is presented as absorbance at 590 nm. Data are mean ± SEM (n = 3) of a typical experiment; similar results were obtained in at least 3 separate experiments.