The effect of different kininogen forms on endothelial cell adhesion to VN.

(A) BRECs were allowed to adhere to VN in the absence or presence of various concentrations of HK (filled circles), HKa (open circles), domain 3 (filled squares), domain 5 (open squares), and domain 6 (filled triangles), and the extent of adhesion is presented as percentage of control. Data are mean ± SEM (n = 3) of a typical experiment, and similar results were observed in at least 3 different experiments. (B) After cells had adhered to VN for 1 hour, HK (open circles), HKa (filled circles), or cRGDfV (filled squares) (10 μg/mL each) was added, and remaining adherent cells after various times were quantitated (presented as absorbance at 590 nm). Data are mean ± SEM (n = 3) of a typical experiment, and similar results were obtained in 3 separate experiments.