Binding of VN to different immobilized kininogen isoforms.
(A) The binding of 2-μg/mL VN to immobilized HK, HKa, GST-D3, GST-D5, GST-D6, or GST (each 5 μg/mL) in the absence (filled bars) or the presence (hatched bars) of 50 μmol/L ZnCl2was carried out, and specific binding is presented as absorbance at 405 nm. Data are mean ± SEM (n = 3) of a typical experiment, and similar results were obtained in 3 separate experiments. (B) The binding of VN to immobilized HKa (filled bars) or domain 5 (hatched bars) was performed in the absence (−) or presence of 10 μg/mL heparin, 10 μg/mL cRGDfV, 200 nmol/L PAI-1, 10 μg/mL GST-VN (1-77), or 10 μg/mL GST in buffer containing 50 μmol/L ZnCl2. Specific binding of VN is presented as percentage of control (binding of VN to HKa or D5 in the absence of any competitor). Data are mean ± SEM (n = 3) of a typical experiment, and similar results were obtained in 3 separate experiments.
