Fig. 5.
Fig. 5. Presence of IgM and IgG mRNA in 14-day B-cell cultures of BMT recipients and healthy subjects. / cDNA from B-cell cultures from 9 recipients and 7 healthy subjects was amplified with the use of 5′ primers specific for VH3 and 3′ primers specific for either IgM or IgG. Results are representative of analysis of triplicate cultures. As an internal control, β-actin mRNA was used. Lanes M were amplified with the use of Cμ primers, lanes G with the use of Cγ primers. (A) BMT recipients. (B) Healthy subjects.

Presence of IgM and IgG mRNA in 14-day B-cell cultures of BMT recipients and healthy subjects.

cDNA from B-cell cultures from 9 recipients and 7 healthy subjects was amplified with the use of 5′ primers specific for VH3 and 3′ primers specific for either IgM or IgG. Results are representative of analysis of triplicate cultures. As an internal control, β-actin mRNA was used. Lanes M were amplified with the use of Cμ primers, lanes G with the use of Cγ primers. (A) BMT recipients. (B) Healthy subjects.

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