Inhibitory effect of increasing concentrations of LY294002 on EPO-induced proliferation and anti-apoptosis in UT-7/EPO cells.

UT-7/EPO cells were plated at a density of 10 000 cells/well in IMDM supplemented with 5% FCS and cultured with increasing concentrations of LY294002 in the presence of 1 U/mL of EPO. MTT reduction assay (A) and cell counting (B) were performed after 3 days of culture. Cell viability was assessed by trypan blue dye exclusion (B). The values represent the mean ± SD from triplicate cultures. (C) Induction of apoptosis by LY294002. UT-7/EPO cells were cultured with 100 μmol/L of LY294002 in the presence of EPO (1 U/mL). Three days later, the cells were harvested and stained with annexin-V-FITC for detection of apoptotic cells (Apo) using FACScan.