American Society of Hematology

Fig. 4.

$Fig. 4. Association of p85 with GP Ib. / (A) One milliliter cytosol, actin cytoskeletal (actin CSK), or membrane cytoskeletal (membrane CSK) platelet subcellular fractions from thrombin-stimulated platelets (2 × 109/mL platelets) or (B) vWF- and botrocetin-stimulated platelets were immunoprecipitated using 5 μL anti-glycocalicin antibody or nonimmune sera (Non I) (5 μL) and immunoblotted using antibody to the p85 subunit of PI 3-kinase. This figure is representative of 4 similar experiments.$

Association of p85 with GP Ib.

(A) One milliliter cytosol, actin cytoskeletal (actin CSK), or membrane cytoskeletal (membrane CSK) platelet subcellular fractions from thrombin-stimulated platelets (2 × 10⁹/mL platelets) or (B) vWF- and botrocetin-stimulated platelets were immunoprecipitated using 5 μL anti-glycocalicin antibody or nonimmune sera (Non I) (5 μL) and immunoblotted using antibody to the p85 subunit of PI 3-kinase. This figure is representative of 4 similar experiments.

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