Regulation of AMUGs and AMDGs by AML1-MTG8 deletion mutants.

(A) Schematic representation of the structures of AML1-MTG8 and AML1-MTG8 deletion mutants Δ538 and Δ487. Runt homology domain (Runt), proline-rich regions (P), and Nervy homology regions (numbered 1-4) are indicated. (B) Differential display patterns of 3 cDNA fragments in control (LNSX) and AML1-MTG8-, Δ538-, and Δ487-expressing L-G cells. AML1-MTG8, Δ538, and Δ487 were introduced on an LNSX vector by retrovirus infection, and control cells were infected with a mock vector. The expression of L1C480 and P10A370 was altered, but the expression of D7T390 was not altered by the NHR2-containing mutant Δ538. Each fragment is indicated by an arrow. (C) Northern blotting analysis of the expression of 3 AMUGs and 2 AMDGs in control and AML1-MTG8-, Δ538-, and Δ487-expressing L-G cells. The membranes were hybridized with the corresponding cDNA fragments isolated by the differential display analysis. Human G3PDH cDNA was used as a control probe. The expression of mast cell carboxypeptidase A, TIS11b, Pim-2, and granzyme B was altered, but the expression of Grb10 was not altered by Δ538.