Overexpression of AML1a.

Overexpression of AML1a induced G-CSF–dependent proliferation of L-G cells. (A) G-CSF–dependent growth of control (LNSX and LNCX) and AML1-MTG8-, AML1b-, and AML1a-expressing L-G cells. Three infections were independently performed for each construct. These infected cells were separately cultured in the presence of 10 ng/mL G-CSF instead of IL-3. The averages of these 3 experiments are shown. Error bars indicate standard deviations. (B) Western blotting analysis of the expression of AML1-MTG8, AML1b, and AML1a in the infected L-G cells. Each protein was detected with anti-HA antibody. The positions of bands of AML1-MTG8, AML1b, and AML1a are indicated on the right.