Flow cytometric analysis of platelets from a patient with Bernard-Soulier syndrome (BSS).

Analysis was performed on whole blood with monoclonal antibodies (mAbs) against glycoprotein (GP) IIb-IIIa (A), GPIbα (B), GPIX (C), and GPV (D). As expected BSS patients' platelets (BSP), which are larger than normal, this patient's platelets (clear area) showed an increase in surface fluorescence compared with platelets from healthy controls (CTL, shaded area) in reaction to the anti-GPIIb-IIIa mAb LYP18. There was no detectable GPIbα (B) or GPIX (C) on the platelets of the patient (clear area), whereas both were present on control platelets (shaded area).