Fig. 5.
Fig. 5. Western blot analysis of plasma GPIb. / Platelet-poor plasma from 2 healthy volunteers and the patient with BSS was immunoprecipitated with antibody AP1, analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) on a 4% to 20% gradient gel in the presence of β-mercaptoethanol, and immunoblotted with anti-GPIbα monoclonal antibody MCB142.11. The glycocalicin-positive control (3.8 ng) is shown in lane 1; BSS plasma is shown in lane 2. The control plasma samples were diluted (1:4) before SDS-PAGE to avoid overloading of the gel (lanes 3 and 4). There was less soluble GPIbα in the plasma from the patient with BSS.

Western blot analysis of plasma GPIb.

Platelet-poor plasma from 2 healthy volunteers and the patient with BSS was immunoprecipitated with antibody AP1, analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) on a 4% to 20% gradient gel in the presence of β-mercaptoethanol, and immunoblotted with anti-GPIbα monoclonal antibody MCB142.11. The glycocalicin-positive control (3.8 ng) is shown in lane 1; BSS plasma is shown in lane 2. The control plasma samples were diluted (1:4) before SDS-PAGE to avoid overloading of the gel (lanes 3 and 4). There was less soluble GPIbα in the plasma from the patient with BSS.

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