Apoptotic alterations among the fractionated cell populations of activated T lymphocytes recovered 2 hours after their exposure to CD2 and CD95 apoptotic stimuli.
(A) ▪---▪ indicates percent cells with high ΔΨm as measured in each fraction with DIOC63; ▿---▿, percent cells with condensed nuclei as visualized by DAPI staining; □---□, percent hypodiploid cells; ▴---▴, percent annexin-V-positive cells. Values are means ± SD of 20 determinations in the CD2 apoptotic system and of 10 determinations in the CD95 system. B) Effect of 50 μmol/L BOC-D.fmk on the occurrence of ΔΨm disruption in F5 cells of the CD2 system and in F2-II cells of the CD95 system. Data shown are representative of 3 different experiments. (C) Propidium iodide staining of DNA from ethanol-permeabilized cells. Values indicate the percentages of hypodiploid cells (linear scales are represented). (D) Total lysates of 5 × 105 cells were analyzed by Western blot using an anti-PARP mAb. Experiments are representative of 6 independent determinations.
