American Society of Hematology

Fig. 7.

$Fig. 7. Characteristics of activated T cells subjected for 2 hours to 100 nmol/L staurosporine. / (A) The ultrastructural changes seen in pooled F4 and F5 cells treated with staurosporine (STS), that is, cell shrinkage and partial chromatin condensation, persist in the presence of BOC-D.fmk. (B) Absence of large-scale DNA fragmentation in F4 and F5 cells contrasting with the approximate 50-kbp sized fragments seen in the F5 fraction of the CD2 system. 1, F2-I; 2, F4; 3, F5; 4, F5 generated after a 2-hour exposure to anti-CD2 (positive control). (C) Confocal immunofluorescence analysis of the localization of cytochrome c and AIF demonstrating that only mitochondrial AIF has been translocated to the cytosol and the nucleus.$

Characteristics of activated T cells subjected for 2 hours to 100 nmol/L staurosporine.

(A) The ultrastructural changes seen in pooled F4 and F5 cells treated with staurosporine (STS), that is, cell shrinkage and partial chromatin condensation, persist in the presence of BOC-D.fmk. (B) Absence of large-scale DNA fragmentation in F4 and F5 cells contrasting with the approximate 50-kbp sized fragments seen in the F5 fraction of the CD2 system. 1, F2-I; 2, F4; 3, F5; 4, F5 generated after a 2-hour exposure to anti-CD2 (positive control). (C) Confocal immunofluorescence analysis of the localization of cytochrome c and AIF demonstrating that only mitochondrial AIF has been translocated to the cytosol and the nucleus.

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