p53-dependent transcription determined by luciferase activity of reporter plasmid pG¹³PYluc.

(A) Jurkat cells stably expressing ZEBRA (Z+) and control cells (Z−) were transiently transfected with 200 ng of reporter plasmids (pG¹³PYluc or pGM¹⁵PYluc) and with 500 ng of p53 expression plasmids for either p53W or p53C. Significant transcription activation was detected with pG¹³PYluc reporter, but not with mutated pGM¹⁵PYluc reporter in Z+ cells with cotransfection of 53W (*P < .05) or 53C (**P < .05). In the absence of cotransfected p53, transcription of p53 reporter genes was extremely low in both Z+ and Z− cells. (B) Parental Jurkat cells were transiently transfected with ZEBRA (500 ng) and p53 expression plasmids (500 ng) with reporter plasmids (200 ng). Transcription of pG¹³PYluc with cotransfection of p53W was slightly increased by transient cotransfection of pSV2-neo-WZhet ZEBRA expression plasmid (Z+) compared to transient transfection of control plasmid pSV2-neo (Z−), but this increase was not significant (P > .05) in pooled experimental data. Transcription of mutated pGM¹⁵PYluc reporter was not activated by transient transfection of either p53 or ZEBRA.