Tumor T-cell line Pno and patient PBLs showed a uniqueTCR-β transcript corresponding to Vβ22/Jβ1.2.

CDR3 size analysis of TCR-Vβ22 transcripts in (A) Pno tumor cells and (B) patient PBLs were analyzed with fluorescent Cβ and Jβ1.2. We amplified cDNA made from total RNA extracted from Pno and PBLs in a PCR reaction primed by Vβ22- and Cβ-specific primers. The unlabeled amplification products were elongated with nested fluorescent Cβ and Jβ1.2. The aliquots were subjected to electrophoresis and analysis on an automated sequencer.