Fig. 4.
Surface plasmon resonance analysis of the binding of IF–Cbl flowing along BIAcore CM5 sensor chips with immobilized wild-type or FM1 mutant form of CUB 5-8.
(A) The IF-Cbl binding to the wild-type CUB 5-8 protein with a flow concentration of 10, 20, and 50 nmol/L ligand. (B) The IF–Cbl binding to the FM1 mutant CUB 5-8 protein with a flow concentration of 10, 20, and 50 nmol/L ligand. The BIA evaluation 3.0 software showed the best fit to a 1-site model with the following binding parameters: wild-type CUB 5-8: Kd = 2 nmol/L; kon = 1.3 × 105mol/L−1s−1; koff= 3.0 × 10−4s−1; FM1 mutant CUB 5-8: Kd= 10 nmol/L; kon = 4.2 × 10.4 mol/L−1 s−1;koff = 4.1 × 10−4s−1.
