Fig. 3.
Recruitment of SHP-1 phosphatase by CD33.
Mo7e, U937, and THP-1 cells (1 × 107/point) were treated with the phosphatase inhibitor pervanadate as indicated and lysed in Triton X-100 lysis buffer, and the cleared lysates immunoprecipitated with anti-CD33 or control antibody (CD4). The immunoprecipitates were blotted with SHP-1 antibody. WCL indicates the presence of SHP-1 in whole cell lysate (1 × 105cells).
