Fig. 5.
Peptide capture of SHP-1 and SHP-2.
Biotinylated peptides spanning tyrosines Y340 or Y358 were linked to streptavidin sepharose beads and used to capture proteins from cell lysates of U937 (1 × 107). Precipitated proteins were blotted with antibodies to SHP-1 or SHP-2. Nonphosphorylated and phosphorylated peptides (denoted as −P*) spanning Y340, Y358, or control TcRζ phosphopeptide were used as indicated. WCL = whole cell lysate (1 × 105).
