Fig. 6.
Fig. 6. The amino SH2 domain of SHP-1 binds Y340 of CD33. / SHP-1 or SHP-1 carrying point mutations within either the amino SH2 domain (SHP-1R30K) or carboxyl SH2 domain (SHP-1R136K) was expressed in 293T cells. Equal amounts of each isoform of SHP-1 was then incubated with phosphopeptide-loaded beads as defined in Figure 5. Bound SHP-1 was eluted with sample buffer and detected by immunoblot. WCL = whole cell lysate demonstrating equal availability of SHP-1.

The amino SH2 domain of SHP-1 binds Y340 of CD33.

SHP-1 or SHP-1 carrying point mutations within either the amino SH2 domain (SHP-1R30K) or carboxyl SH2 domain (SHP-1R136K) was expressed in 293T cells. Equal amounts of each isoform of SHP-1 was then incubated with phosphopeptide-loaded beads as defined in Figure 5. Bound SHP-1 was eluted with sample buffer and detected by immunoblot. WCL = whole cell lysate demonstrating equal availability of SHP-1.

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