American Society of Hematology

Fig. 3.

$Fig. 3. MADDAM mRNA expression in MAC compared with immature and mature DCs on day 7 of culture. / MOs were cultured with either human serum to induce MAC differentiation or FCS + IL-4, FCS + GM-CSF, and FCS + IL-4 + GM-CSF, respectively, to induce DC differentiation (A). In addition, MOs were cultured under serum-free conditions with IL-4 + GM-CSF with or without the addition of TNF-α on day 5 (B). In (C), DCs were generated from either MNCs or different elutriation fractions containing MOs with IL-4 + GM-CSF + FCS and the addition of TNF-α on day 5. In (D), MOs into MAC differentiation under serum-free conditions with 100 ng/mL M-CSF was switched to the DC differentiation pathway on day 4 by culture with FCS, IL-4, and GM-CSF or the other way around. Total RNA was extracted and the Northern blot analysis was performed as described in “Materials and methods.” As a control for RNA loading, the membrane was rehybridized with an 18S rRNA-oligonucleotide (A-D).$

MADDAM mRNA expression in MAC compared with immature and mature DCs on day 7 of culture.

MOs were cultured with either human serum to induce MAC differentiation or FCS + IL-4, FCS + GM-CSF, and FCS + IL-4 + GM-CSF, respectively, to induce DC differentiation (A). In addition, MOs were cultured under serum-free conditions with IL-4 + GM-CSF with or without the addition of TNF-α on day 5 (B). In (C), DCs were generated from either MNCs or different elutriation fractions containing MOs with IL-4 + GM-CSF + FCS and the addition of TNF-α on day 5. In (D), MOs into MAC differentiation under serum-free conditions with 100 ng/mL M-CSF was switched to the DC differentiation pathway on day 4 by culture with FCS, IL-4, and GM-CSF or the other way around. Total RNA was extracted and the Northern blot analysis was performed as described in “Materials and methods.” As a control for RNA loading, the membrane was rehybridized with an 18S rRNA-oligonucleotide (A-D).

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