Comparison of MCP-1 and MIP-1 levels in the lung with frequencies of host monocytes and donor T cells.
▪ indicates host monocytes; •, donor T cells. B10.BR recipient mice were preconditioned with TBI (7.5 Gy, day −1) and Cy (120 mg/kg per day, days −3, −2) and given C57BL/6 BM with 15 × 106 spleen cells (BMS) on the day of BMT (day 0). Chemokine levels in picograms per milliliter of BAL fluid (in shaded box) were determined by ELISA of lung protein extracts as described in “Materials and methods” from mice killed on day −3 (prior to Cy administration), day 0 (prior to BMT), day 3, and day 7 after BMT. Frequencies of host monocytes and donor T cells (as a percentage of total nucleated cells) were determined by immunohistochemical staining of lung cryosections using biotinylated monoclonal antibodies as described previously.6
