Fig. 2.
STI 571 inhibits autophosphorylation of an activated mutant form of c-kit.
HMC-1 cells were treated with various concentrations of STI 571 for 90 minutes. Whole cell lysates were prepared and immunoblotted with an anti-P-Tyr monoclonal antibody. The membrane was stripped and reprobed with a monoclonal antibody specific for human c-kit. The arrow indicates the position of the 145-kd isoform of c-kit. Representative results from 1 of 8 independent experiments are shown.
