STI 571 has no effect on GM-CSF–dependent proliferation of M-07e cells.

Serum was withheld from M-07e cells overnight before they were used in proliferation assays. Cells were plated in a 96-well plate at a concentration of 50 000 cells/well and grown in RPMI-1640 supplemented with 10% FCS with and without 200 U/mL GM-CSF. STI 571 was added at the same time as GM-CSF. Proliferation at 48 hours was measured with an XTT-based assay system. Results are expressed as the percentage of maximal proliferation (GM-CSF only) (± SD). Representative results from 1 of 2 independent experiments are shown.