American Society of Hematology

Fig. 3.

$Fig. 3. Comparison of CD4/CD3-ζ DNA copy number obtained from PBMC and lymphoid tissue by quantitative competitive PCR. / (A) PBMC sample (142 copies/million cells) and (B) lymph node purified CD8+ cell fraction (below the limits of quantitation) obtained from 1 patient 10 weeks after an infusion of 109CD4/CD3-ζ–modified CD8+ T cells. Similar data are shown in panel C for PBMC (> 5000 copies/million cells) and in panel D for unfractionated mononuclear cells (MC) from tonsillar tissue (567 copies/million cells) for a different patient 2 weeks after an infusion of 1010 CD4/CD3-ζ–modified CD4+ and CD8+ T cells. The e+ cell line, which contains 10 copies of the CD4/CD3-ζ gene with a 102-base-pair DNA insert per cell, was used for standardization.$

Comparison of CD4/CD3-ζ DNA copy number obtained from PBMC and lymphoid tissue by quantitative competitive PCR.

(A) PBMC sample (142 copies/million cells) and (B) lymph node purified CD8⁺ cell fraction (below the limits of quantitation) obtained from 1 patient 10 weeks after an infusion of 10⁹CD4/CD3-ζ–modified CD8⁺ T cells. Similar data are shown in panel C for PBMC (> 5000 copies/million cells) and in panel D for unfractionated mononuclear cells (MC) from tonsillar tissue (567 copies/million cells) for a different patient 2 weeks after an infusion of 1010 CD4/CD3-ζ–modified CD4⁺ and CD8⁺ T cells. The e⁺ cell line, which contains 10 copies of the CD4/CD3-ζ gene with a 102-base-pair DNA insert per cell, was used for standardization.

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