Cytofluorometric profiles of DC permeabilization induced by ATP.

(A) Forward-scatter (FSC) and side-scatter (SSC) dot plot. R1 represents the DC population that was gated for analysis, thus excluding cellular debris and dead cells. DCs were incubated with 10 μmol/L ethidium bromide (B) or 10 μg/mL YO-PRO 1 (C) and treated (ATP) or not (Ct) with 5 mmol/L ATP for 15 minutes at 37°C.