The ectonucleotidase inhibitor, 5′FSBA, enhances ATP-mediated DC permeabilization.

The DC suspension was previously treated with 1 mmol/L of 5′FSBA for 1 hour at 37°C. These cells were then washed twice and incubated with ATP (5 mmol/L) and ethidium bromide (10 μmol/L) for 15 minutes at 37°C. The degree of permeabilization was analyzed by flow cytometry. Data shown were normalized and compared by Studentt test. *Results from DCs treated with 5′FSBA were significantly different from those of untreated cells (P < .001). Data are the average of 3 experiments (mean ± SD).