Fig. 9.
Oxidized ATP treatment blocks the ATP-mediated DC permeabilization.
DC suspensions were pretreated with different concentrations of oxidized ATP (oATP) for 2 hours at 37°C. These cells were subsequently treated with ATP (5 mmol/L) plus ethidium bromide (10 μmol/L) and incubated for 15 minutes more. The permeabilization inhibition was analyzed by flow cytometry.