P2ZP2X7 activation-dependent apoptosis of DCs.
(A) DCs were incubated with UTP, BzATP, or ATP (all at 5 mmol/L) for 30 minutes at 37°C; washed twice with PBS/BSA; and incubated additionally for 6 hours in complete RPMI 1640 medium. Alternatively, DCs were incubated with 500 μmol/L of the P2Z/P2X7 antagonist, oATP, for 2 hours before ATP (5 mmol/L) treatment. The DCs treated with oATP plus ATP (oATP + ATP) were compared with the control untreated DCs (Control) and with those treated with ATP alone (5 mmol/L). Data are based on at least 3 independent experiments. The results (mean ± SD) were compared with Student t test. *Data from DCs treated with ATP or BzATP were significantly different from control (P < .001); **data from ATP-treated DCs submitted to oATP inhibition were significantly different from ATP-treated DCs (P < .001). (B) Dose-response curve of ATP-induced apoptosis. Apoptosis was ascertained by the TUNEL technique and flow cytometry. Data are based on 3 independent experiments (mean ± SD).
