Anti-CD11c antibody labeling of apoptotic and nonapoptotic DCs.

The DCs were treated with 5 mmol/L ATP for 30 minutes, washed, and incubated for an additional 6 hours. These cells were then labeled with the anti-CD11c mAb before the detection of apoptosis by TUNEL. The dot plots show the double labeling for CD11c (PE) and TUNEL (FITC) of either control cells (Ct) (upper panel) or those treated with ATP (ATP) (lower panel).